Ured za
istraživanje


PROJEKTI
   

Project
Acronym: Biotransformations of industrial interest 
Name: Study on biotransformations of industrial interest in aqueous and non-conventional media 
Project status: From: 2007-01-01 To: 2008-12-30 (Completed)
Type (Programme): BILAT 
Project funding: -
International partner
Organisation Name: University of Pannonia, Research Institute for Chemical and Process Engineering  
Organisation adress: H-8200 Veszprem Egyetem u. 10 
Organisation country: Hungary 
Contact person name: Dr. Gubicza Laszlo 
Contact person email: Email 
Croatian partner
Organisation name: Fakultet kemijskog inženjerstva i tehnologije 
Organisation address: Marulićev trg 19 
Contact person name: Prof.dr.sc. Đurđa Vasić-Rački
Contact person tel:
++385 1 4597104  Contact person fax: ++385 1 4597133 
Contact person e-mail: Email 
Short description of project
The aim of biotransformation research is to develop the continuous processes to produce industrially important compounds. The chemical engineering concept in biotransformation research is to design optimal reactors for this purpose by integration of the biotransformation, biocatalyst, bioreactor type and down-stream processing. The bioreactor should be designed such that the specific biological and technological demands of a process are met. Successful scale-up of biotransformation requires a good understanding of the interactions between the biocatalysts and the chemical and physical environments in the reactor. Characteristics of the biotransformation are used as the basis for evaluating a range of media, the need for process additives and together with kinetic and stability constraints form the criteria for reactor selection. The reaction medium, the kinetic and biocatalyst constraints determine the bioreactor mode of operation and liquid flow pattern. An important feature in biotransformation is the solvent. The biocatalysts mostly require an aqueous surrounding, while the target compounds prefer organic solvents. Therefore, non-conventional media (two phases, gas phase, ionic liquid, supercritical fluid etc.) are of great interest. The aim of the project is to examine several biotransformation of industrial interest notably those focusing on the continuous synthesis of fine chemicals or intermediates using stereospecific biocatalysts. These reactions are to be investigated in aqueous and non-conventional media. The overall objective of the project is to demonstrate two main technologies a) Use of isolated enzymes with coenzyme regeneration and b) Use of enzymes in the whole cells. The following biocatalysts will be investigated: 1. hydrolases(proteinase, keratinese)          2. dehydrogenases (ADH). These enzymes need coenzymes NAD(P)H as redox alcohol equivalents, which have to be regenerated and 3. oxidases The investigation will be focused on the increase of operational stability of the biocatalysts for the use in continuous process as well as to suitable system for the coenzyme regeneration will be investigated.  
Short description of the task performed by Croatian partner
Cilj istraživanja biotransformacija je razviti kontinuirane procese za  proizvodnju industrijski važnih spojeva. Koncept kemijskog inženjerstva ovih istraživanja je dizajn optimalnih reaktora s integriranim biotransformacijama, biokatalizatorima, tipovima bioreaktora i procesima separacija. Bioreaktor treba biti osmišljen tako da zadovolji specifičnosti biološkog i tehnološkog zahtjeva procesa. Uspješnost  scale-up biotransformacije  zahtijeva dobro razumijevanje interakcija  između biokatalizatora i kemijskih i fizikalnih okruženja u reaktoru. Karakteristike biotransformacija se koriste kao osnova za vrednovanje različitih medija, potrebe za procesnim  aditivima, te  zajedno s tim, uz  ograničenja kinetičke  stabilnosti, formirati kriterije za izbor reaktora. Reakcijska, kinetička i biokatalitička ograničenja određuju a način rada bioreaktor a i njegova hidrodinamička svojstva.  Važna značajka biotransformacija je otapalo. Biokatalizatori  najčešće  zahtijevaju vodeno okružuje, a ciljani spojevi organsko. Zbog  toga, ne-konvencionalni mediji  (dvije faze, faze plina, ionske tekućine, superkritične tekućine) su vrlo interesantni Cilj projekta je ispitati nekolikoindustrijski zanimljivih  biotransformacija posebno onih s naglaskom na kontinuiranu sintezu finih kemikalija ili intermedijera koje  koriste stereospecifičnost biokatalizatora  u nekonvencionalnim medijima. Opći cilj projekta je pokazati dvije glavne tehnologije) upotreba izoliranih enzima s regeneracijom koenzima i B) upotreba enzima u cijelim stanicama. Istraživat će se slijedeći bio0katalizatori: Hidrolaze (proteinaze, keratinaze) Dehidrogenaze (ADH). Ovi enzimi trebaju koenzim kao redoks ekvivalent, koji će biti regeneriran. Oxidase Istraživanja će biti usmjerena na povećanje operacijskej stabilnosti biokatalizatora u kontinuiranom procesu, kao i odgovarajućeg sustava za regeneraciju koenzima..  


   

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